Illumina Paired-End Module

Paired-End Module


Applications: DNA Sequencing, Gene Regulation Analysis, Sequencing-Based Transcriptome Analysis, SNP Discovery and Structural Variation Analysis, Cytogenetic Analysis, DNA-Protein Interaction Analysis (ChIP-Seq), Sequencing-Based Methylation Analysis, Small RNA Discovery and Analysis


The Paired-End module enables paired-end sequencing up to 2 x 100 bp for fragments ranging from 200 bp to 5 kb. Paired-end sequencing data offers long-range positional information, empowers easy detection of structural variations such as chromosomal rearrangements, copy number variations, and indels; and simplifies de novo assembly. The Paired-End Module and Genome Analyzer can generate reads from both ends of the library templates in less than a week. More...


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System Description

The Paired-End Module is a fluidics station that attaches to the Genome Analyzer. After resysthesis of the reverse strand, the original forward strand is cleaved away and the complementary strands are bridge amplified to form new clusters for the second read.

HIGHLIGHTS

Simplest Paired-End Workflow

Use a simple protocol to create paired-end gDNA libraries in ~3 hours hands-on time and ~6 hours total time

Lowest Sample Requirements

use ≤ 1 µg of DNA to generate human genomic paired-end DNA libraries

Discover the Broadest Range of Genomic Variation

Only next-generation sequencing platform with the unique combination of short- and long-insert paired-end sequencing libraries to discover all variations

Workflow and Specs

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Paired-End Module Kits

Genome Analyzer System Warranties

Genome Analyzer System Warranties

A comprehensive 12 month warranty covering all hardware and software includes unlimited business hours emergency on-site service calls; a preventative maintenance visit; parts, labor, and consumables, and unlimited phone support and assistance. After the first year there is premium, plus, basic, or parts only options to extend warranty coverage.

Additional Details

SV-301-1004
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Illumina Sequencing Kits v4

Illumina Sequencing Kits v4

The Illumina Sequencing Kit v4 contains ready-to-load reagents for accurately determining the DNA sequence of each cluster on a flow cell using Sequencing by Synthesis technology and the Genome Analyzer. The improved chemistry of this kit enable researchers to conduct much longer, high-accuracy, sequencing runs than previous versions.

Additional Details

FC-104-4001
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Small RNA Sample Prep Kit

Small RNA Sample Prep Kit

With Small RNA Discovery and Analysis, Illumina scientists have designed a sample preparation protocol that enables sequencing of of known and novel microRNAs as well as other non-coding RNAs, such as piwi-interacting RNAs and siRNAs. Sequencing-based Small RNA Sample Prep Kits offer researchers the flexibility to select the length of the small RNA they wish to investigate, enabling size-focused or broad size range investigation of different classes of small RNA on one universal platform.

Additional Details

FC-102-1009
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Mate Pair Library Prep Kit

Mate Pair Library Prep Kit

The Mate Pair Library Prep Kit includes reagents needed to generate libraries with inserts from 2-5 kb in size. Prepared libraries consist of short fragments made up of two segments that were originally separated by several kilobases in the genome. Mate Pairs are sequenced using a two-adapter strategy identical to the paired-end sequencing method.

Additional Details

PE-112-1002
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Illumina Sequencing Kits v3

Illumina Sequencing Kits v3

The Illumina Sequencing Kit v3 contains ready-to-load reagents for accurately determining the DNA sequence of each cluster on a flow cell using Sequencing by Synthesis technology and the Genome Analyzer. The improved chemistry of this kit enable researchers to conduct much longer, high-accuracy, sequencing runs than previous versions.

Additional Details

FC-104-3001
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Multiplexing Sample Preparation Oligonucleotide Kit

Multiplexing Sample Preparation Oligonucleotide Kit

The Multiplexing Sample Preparation Oligonucleotide Kit contains 12 unique oligonucleotides to “tag” libraries for pooling in one flow cell lane. Up to 96 samples can be sequenced on a single flow cell using the Genome Analyzer. The multiplex sequencing process is fully automated using the Cluster Station, Genome Analyzer, and Paired-End Module.

Additional Details

PE-400-1001
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Multiplexing Sequencing Primers and PhiX Control Kit V1

Multiplexing Sequencing Primers and PhiX Control Kit V1

The Multiplexing Sequencing Primers and PhiX Control Kit V1 is used to sequence samples tagged by using the Multiplexing Sample Preparation Oligonucleotide Kit and then amplified in the Cluster Station. Sequencing is fully automated on the Genome Analyzer.

Additional Details

PE-400-1002
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Paired-End Cluster Generation Kit V2

Paired-End Cluster Generation Kit V2

The Paired-End Cluster Generation Kit V2 provides reagents that bind samples to complementary adapter oligos on the flow cell. These enable copying of the DNA strand after the first sequencing run; the copied strand is used to sequence the other fragment end. The Cluster Station amplifies attached DNA fragments to clonal clusters of around 1000 copies each.

Additional Details

PE-103-2001
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Standard Cluster Generation Kit v2

Standard Cluster Generation Kit v2

The Standard Cluster Generation Kit v2 provides ready-to-load reagents that bind DNA library samples to complementary adapter oligos grafted on the flow cell surface. The master-mixed reagents in this kit provide a streamlined workflow with a total of 10 minutes hands-on time for reagent preparation and setup.

Additional Details

FC-103-2001
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Paired-End DNA Sample Prep Kit V1

Paired-End DNA Sample Prep Kit V1

The Paired-End DNA Sample Prep Kit helps build DNA libraries for paired-end sequencing with insert sizes from 200-500 bp. Preparation includes introduction of two unique sequencing primer binding sites and adapter sequences to the anti-parallel strands of the polished whole-genome DNA fragments followed by gel-based size selection and purification.

Additional Details

PE-102-1001
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Standard Cluster Generation Kit v4

Standard Cluster Generation Kit v4

The Standard Cluster Generation Kit v4 provides ready-to-load reagents that bind DNA library samples to complementary adapter oligos grafted on the flow cell surface. The master-mixed reagents in this kit provide a streamlined workflow with a total of 10 minutes hands-on time for reagent preparation and setup.

Additional Details

GD-103-4001
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Paired-End Cluster Generation Kit V4

Paired-End Cluster Generation Kit V4

The Paired-End Cluster Generation Kit V4 provides reagents that bind samples to complementary adapter oligos on the flow cell. These enable copying of the DNA strand after the first sequencing run; the copied strand is used to sequence the other fragment end. The Cluster Station amplifies attached DNA fragments to clonal clusters of ~1000 copies each.

Additional Details

PE-203-4001
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cBot Paired-End Cluster Generation Kit

cBot Paired-End Cluster Generation Kit

The cBot Paired-End Cluster Generation Kit provides reagents that bind samples to complementary adapter oligos on the flow cell. These enable copying of the DNA strand after the first sequencing run; the copied strand is used to sequence the other fragment end. The cBot amplifies attached DNA fragments to clonal clusters of ~1000 copies each.

Additional Details

PE-300-1001
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cBot Single Read Cluster Generation Kit

cBot Single Read Cluster Generation Kit

The cBot Single Read Cluster Generation Kit provides reagents that bind DNA library samples to complementary adapter oligonucleotides grafted on the flow cell surface. The pre-mixed, 96-well plate format reagents in this kit provide a streamlined workflow with no reagent preparation and a total of 10 minutes hands-on time.

Additional Details

GD-300-1001
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Small RNA Cluster Generation Kits

Small RNA Cluster Generation Kits

For Digital Gene Expression (DGE): Small RNA Discovery and Analysis, the Small RNA Cluster Generation Kit is used to bind total RNA samples to complementary adapter oligos on a flow cell surface. The Cluster Station isothermally amplifies attached cDNA and resulting high-density template cluster array is sequenced by the Illumina Genome Analyzer.

Additional Details

FC-103-1008
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mRNA-Seq 8-Sample Prep Kit

mRNA-Seq 8-Sample Prep Kit

The mRNA-Seq 8-Sample Prep Kit is used to build DNA libraries for single-read and paired-end sequencing on the Genome Analyzer. Sample preparation is straightforward, using standard molecular biology techniques and requiring minimal hands-on time. mRNA template libraries are sequenced just as any DNA sample using Illumina Sequencing.

Additional Details

RS-100-0801
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ChIP-Seq DNA Sample Prep Kit

ChIP-Seq DNA Sample Prep Kit

Chromatin immunoprecipitation (ChIP) selectively finds DNA sequences bound by a particular protein. The ChIP-Seq DNA Sample Prep Kit helps build DNA libraries. The ChIP process enriches specific crosslinked DNA protein complexes using an antibody and unique oligonucleotide adapters added to small stretches of DNA bound to the protein of interest.

Additional Details

IP-102-1001
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Tag Profiling Sample Prep Kits

Tag Profiling Sample Prep Kits

Tag Profiling Sample Prep Kits are used to build constructs comprised of a unique, positionally known 20- or 21-base pair cDNA tag with defined adapters attached to both ends. Access to any mRNA is through two restriction enzyme tag construction options. Kits are available complete or containing only the required oligonucleotides.

Additional Details

FC-102-1005
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Tag Profiling Cluster Generation Kits

Tag Profiling Cluster Generation Kits

Tag Profiling Cluster Generation Kits are used with total RNA samples to bind the tag construct library to complementary adapter oligos grafted on the flow cell surface. The Cluster Station isothermally amplifies the attached cDNA constructs to create clonal clusters of roughly 1,000 copies each.

Additional Details

FC-103-1004
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Genomic DNA Sample Prep Kits

Genomic DNA Sample Prep Kits

Go from purified genomic DNA to a DNA library. Genomic DNA Sample Prep Kits include reagents to build DNA libraries for single-read sequencing. Random size–selected fragments of DNA are created, polished, and prepared for the addition of unique adapters to fragment ends. Following a short PCR enrichment, the library is ready to load.

Additional Details

FC-102-1001
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Software

Illumina proudly offers system and analysis software designed to maximize your data quality and throughput level. Each instrument includes system control software designed for optimal performance. Data can be analyzed using Illumina's user-friendly GenomeStudio software or a third-party package.

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