SQ Module
Applications: Sequencing, DNA Sequencing, Gene Regulation Analysis, Sequencing-Based Transcriptome Analysis, SNP Genotyping and CNV Analysis, Whole-Genome Genotyping and Copy Number Analysis
The SQ Module allows integration of the high-throughput power of genotyping and gene expression arrays with the flexibility and resolution of next-generation sequencing. It is an add-on device that, in concert with the Genome Analyzer Cluster Station, enables sequencing by synthesis on an existing iScan System. More...
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System Description
The SQ Module provides any lab that has traditionally run microarray assays an economical and convenient gateway to next-generation sequencing. The streamlined workflow ensures convenient operation and reproducible results with minimal user intervention.
The SQ Module enables resequencing of small genomes or targeted genomic regions, a deeper understanding of the transcriptome with mRNA-Seq, discovery and profiling of miRNA expression, characterization of genome-wide DNA-protein interaction sites with ChIP-Seq, and validation of array-based expression and splice variant data, and allows identification of target SNPs from sequencing experiments to design iSelect custom genotyping panels for any species and any study.
- The SQ Module provides flexibility for paired-end reads, multiplexing (up to 12 samples per lane; 96 samples per flow cell), and customizable read lengths for any experimental design.
- To perform sequencing on the iScan System, a Sequencing Module, Cluster Station, and data analysis tools are required.
HIGHLIGHTS
Integrated
Seamlessly combine array and sequencing assays to enable an unmatched range of applications
Flexible
Easily transition between array and sequencing assays
Cost-effective
Conveniently and economically add next-generation sequencing capabilities to your research
High-quality
Reliably generate the highest raw read accuracy and maximal output of perfect reads
Workflow and Specs
Workflow
Streamlined microarray and sequencing workflows allow you to quickly generate high-quality data, conveniently and reliably
Assays/Protocols
BeadArray: Infinium HD, DASL, Direct Hyb
Sequencing: Single-Read Sequencing, Paired-End Sequencing, Multiplex Sequencing, Mate-Pair Library Sequencing, ChIP-Seq Sequencing, mRNA-Seq, Small RNA Discovery and Analysis
| iScan Specifications | |
|---|---|
| Field of View | 1 mm wide |
| Pixel Resolution | 0.54 micron |
| Laser Excitation | 532 nm and 658 nm dual-laser excitation |
| Emission Spectra | 550-600 nm and 677-697 nm |
| Image Registration | Automatic |
| Data Extraction | Automatic |
| Image File Output | TIFF, PNG, or JPG data file output with automatic image quality analysis (uncompressed or compressed) |
| Data File Output | IDAT (Illumina proprietary format) |
| Detector Analog-to-Digital Converter | 16-bit |
| Limit of Detection | 5 fluor/µm2 (Cy3 or Cy5) |
| Input Voltage | 100-240V AC 50/60 Hz, single phase |
| Input Power | 3A (110V); 1.5A (220V) |
| Dimensions | 52 cm (width) × 45 cm (height) × 66 cm (depth) |
| Dimensions of Air Table | 61 cm (width) × 6 cm (height) × 69 cm (depth) |
| Weight | 65 kg |
| iScanSQ Specifications | |
|---|---|
| Width | 61 cm (24 in) |
| Height | 76 cm (30 in) |
| Depth | 71 cm (28 in) |
| Weight | 50 kg (110 lbs) |
Software
Illumina proudly offers system and analysis software designed to maximize your data quality and throughput level. Each instrument includes system control software designed for optimal performance. Data can be analyzed using Illumina's user-friendly GenomeStudio software or a third-party package.
Documentation
- iScan and AutoLoader2 System Guide
- AutoLoader2 Quick Reference Card
- iScan System Quick Reference Card
Literature
Videos
Support
Illumina's Customer Solutions Team is dedicated to providing you with the resources you need to support your research. Scientists specializing in Illumina's technologies, scientific applications, software, and hardware systems are available even before your first system purchase. Field Service Engineers expertly install and qualify your instrument to guarantee a fully functional system. Field Application Scientists offer extensive training, enabling labs to quickly become proficient at generating industry-leading data quality at unparalleled throughput levels. Technical Support Scientists are just a phone call or email away and can help you plan your project, diagnose a problem, and interpret results.
We strive to continuously exceed your expectations, so that you can maximize the impact of Illumina technology in your lab. For more information, visit our Support section