The Illumina GoldenGate Genotyping Assay is a flexible, pre-optimized assay that uses a discriminatory DNA polymerase and ligase to interrogate 96, or from 384 to 1,536, SNP loci simultaneously. The protocol can be performed manually or can be easily automated, with a streamlined workflow that allows a single technician to generate over 300,000 genotypes in just six hands-on hours. GoldenGate Standard Panels include options for human and mouse content. GoldenGate Custom Panels can be designed for many species with user-defined content. GoldenGate Genotyping samples can be conveniently processed on either the Sentrix Universal-16 BeadChip or Universal-96 Array Matrix multi-sample array formats.

GoldenGate assay highlights

• Proven technology: Used in genotyping centers worldwide
• High-quality data: Average call rates > 99%
• Scalable solution: 96, or from 384 to 1,536 SNPs per sample, 16 or 96 samples in parallel
• Streamlined workflow: Manual or automated processing with multiple stopping points
• Flexible content: An expanding selection of standard panels or custom panels with the SNP loci of your choice