Discover and profile the entire universe of mRNA in any eukaryotic species with mRNA-Seq. With no probes or primers to design, mRNA-Seq delivers unbiased and unparalleled information about the transcriptome. The simple workflow and Illumina's optimized mRNA-Seq kits allow you to quickly generate full sequence from any poly-A tailed RNA to analyze novel transcripts, novel isoforms, alternative splice sites, rare transcripts, and cSNPs in one experiment utilizing Illumina's Genome Analyzer system or iScan-Seq.

Sequencing-based mRNA analysis records the numerical frequency of a sequence in the library population, eliminating background signals observed using relative expression profiles generated with microarray hybridization technology. mRNA-Seq offers a new level of assay customizability to optimize each experiment to a study’s specific design and purposes. Coverage depth can be tuned by the user and increased simply by sequencing more of the sample. This customizable depth facilitates the entire range of applications, from expression profiling and sample classification with low read count to rare transcript or variant discovery with deep coverage.

mRNA-Seq Highlights

• Powerful Platform: Sequence full eukaryotic mRNA without prior sequence information
• High Sensitivity: Detect single transcripts per cell in 1 µg of total RNA or less
• Digital Readout: Count the numerical frequency of each sequence in your library population and effectively eliminate background noise
• Tuneable Coverage: Discover rare transcripts with unlimited dynamic range
• Global Validation: Generate genome-wide data comparable to qPCR
• Simple Workflow: Accelerate mRNA expresssion analysis with Illumina's easy-to-use sample prep kits and protocols