Sample Multiplexing is a useful technique when targeting specific genomic regions or working with smaller genomes. Pooling samples into a single lane of a flow cell exponentially increases the number of samples analyzed in a single run without drastically increasing cost or time. To prepare samples for multiplexing, a unique identifier tag, or index, is added to each library. An automated three-read sequencing strategy accurately identifies each uniquely-tagged sample for individual downstream analysis.

Highlights

• Fast, High-Throughput Strategy: Automated sequencing of up to 12 samples per lane or 96 samples per flow cell
• Cost-Effective Method: Multisample pooling improves productivity by reducing time and reagent use
• High-Quality Data: Accurate maintenance of read length of unknown sequences
• Simplified Analysis: Automatic sample identification with index using the Pipeline Analysis software