A simple modification to the standard single-read DNA library preparation facilitates reading both the forward and reverse template strands of each cluster during one paired-end read. In addition to sequence information, both reads contain long range positional information, allowing for highly precise alignment of reads. The unique paired-end sequencing protocol allows the end user to choose the length of the insert (200–500 bp) and sequence either end of the insert, generating highly quality, alignable sequence data. A typical paired-end run can achieve 2 × 75 bp reads and up to 200 million reads.

Highlights

• Simple Paired-End Libraries: Simple protocol workflow allows generation of unique ranges of insert sizes
• Efficient DNA Use: Requires the same amount of DNA as single-read, 100 ng–1 μg gDNA or cDNA
• Broad Range of Applications: Does not require methylation of DNA or restriction digestion; can be used for bisulfite sequencing
• Simplified Data Analysis: Higher quality sequence assemblies with short-insert libraries